What Analytical Packet Should a Research Peptide Standard Include?
A bench-science procurement checklist for peptide reference-standard release: COA, HPLC trace, MS identity, sequence evidence, purity method, counter-ion, water, and storage data.
Published May 26, 2026 · 5 min read · By Lyochem Regulatory Team
A research peptide standard is only useful if the release packet lets a lab defend identity, purity, and handling conditions later. The right packet is not a decorative COA; it is the traceable evidence set that connects the vial on the bench to the sequence, method, and lot history.
What analytical packet should a research peptide standard include?
A research peptide analytical packet should include a batch-specific COA, HPLC chromatogram with integration table, mass-spec identity report, sequence-confirmation evidence where molecular complexity requires it, counter-ion and water content, storage condition, retest date, and method notes. For calibration or publication-critical work, ask for raw HPLC/MS files and impurity-profile explanation.
Minimum packet at receipt
The minimum review set should answer four questions: is this the labelled molecule, how pure is the main peak, what else is in the vial, and how should the lot be stored?
- COA with lot number, manufacture date, retest date, release specification, and signed decision
- RP-HPLC trace with retention time, main-peak area percent, integration threshold, and method conditions
- ESI-MS or LC-MS identity report against theoretical molecular weight
- LC-MS/MS, Edman, or orthogonal sequence evidence for long or sequence-sensitive peptides
- Counter-ion, water content, residual-solvent note, and storage guidance
When to ask for more
For first-time supplier qualification, publication-critical controls, in vivo studies, or method-development standards, request raw chromatogram files, full MS spectra, impurity assignment where available, and a short explanation of any unusual shoulder or retention-time drift. The point is not paperwork volume; it is whether the lab can reproduce and defend the lot's identity later.
Lyochem release posture
Lyochem keeps HPLC and MS evidence tied to the released lot and can extend the packet with sequence-level confirmation or impurity-profile explanation when the buyer's workflow needs it. Use the quote form to specify whether the material is for calibration, assay control, CRO transfer, or general bench research so the analytical scope matches the downstream risk.