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Metastin (45-54) · HPG-axis research peptide
Research documentation
Ask sales for the Kisspeptin-10 batch COA and the analytical evidence needed to qualify the material for your study. Confirm the available purity and identity records, lot continuity, handling information, and method requirements before ordering.
Overview
Kisspeptin-10 (KP-10; Metastin 45-54) is a decapeptide agonist of the KISS1 receptor (GPR54), corresponding to the C-terminal segment shared across the KISS1-gene-encoded kisspeptin family. Despite its short length, it preserves essentially the full GPR54-binding activity of the longer congeners (Kisspeptin-54, -14 and -13). Because kisspeptin acts on hypothalamic neurons to trigger GnRH release — and thereby pituitary LH and FSH secretion — KP-10 serves as the standard probe for interrogating the top of the hypothalamic-pituitary-gonadal (HPG) axis. Lyochem supplies Kisspeptin-10 as a lyophilized reference standard to a ≥99.0% HPLC purity specification, produced by routine SPPS at this chain length. Release testing combines RP-HPLC peak integration with mass-spec identity, water determination and counter-ion analysis; confirmatory LC-MS/MS sequence mapping is available on request. Investigators should account for the peptide's brief plasma residence — on the order of minutes, driven by peptidase cleavage at the C-terminal RFamide — which is why in vivo designs commonly rely on continuous infusion or repeated bolus to sustain HPG-axis drive, with intranasal formats explored as a longer-acting alternative.
Applications & buyer fit
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every lot has its own batch-specific CoA — HPLC purity and MS identity, plus any analytical scope agreed at quote stage — tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Sold for Research Use Only under the receiving laboratory's institutional and jurisdictional regulations. Not a finished dosage form and not labelled for human administration. Several items here are approved prescription hormones and gonadotropins (HCG, HMG, Oxytocin, PT-141 / Bremelanotide) that carry specific import and licensing frameworks in most jurisdictions; buyers are responsible for verifying eligibility for the destination market.
Selected literature
Frequently asked questions
Kisspeptin-10 is confirmed by RP-HPLC for purity and ESI-MS for the expected mass on the batch COA, with LC-MS/MS sequencing available on request to verify the residue order. The C-terminal is amidated (the RFamide motif that binds GPR54), and amidation shifts the mass by roughly 1 Da versus the free-acid form, so the mass method must resolve that difference to confirm the peptide is correctly amidated rather than a free-acid impurity. Because the free-acid form has reduced receptor activity, a reference lot intended for GPR54 work should show a mass and sequence consistent with the amidated C-terminus, not merely the correct residue composition.
Kisspeptin-10 ships as a salt with bound water, so net peptide content (after counter-ion and moisture correction), when reported on the COA, is the figure to dose against for accurate molar concentrations in GPR54 assays, not the gross vial mass. Reconstitute gently to avoid air-liquid-interface denaturation, aliquot into single-use volumes, and keep frozen with minimal freeze-thaw cycling to preserve the chromatographic profile. Because the arginine-rich, amidated peptide can be prone to adsorptive loss at low concentrations, adding carrier protein to dilute working stocks helps keep the delivered concentration consistent with the characterized reference.
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