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p53-HDM2-binding · penetratin-fused research peptide
Research documentation
Ask sales for the PNC-27 batch COA and the analytical evidence needed to qualify the material for your study. Confirm the available purity and identity records, lot continuity, handling information, and method requirements before ordering.
Overview
PNC-27 is engineered as a bifunctional chimera: a p53-derived HDM-2-binding segment (residues 12-26) occupies the N-terminus, while the C-terminus carries a penetratin transduction domain drawn from Antennapedia-homeodomain residues 43-58. Functionally, the penetratin portion ferries the construct across the plasma membrane so the p53 element can contact intracellular HDM-2, the human MDM2 ortholog. Its defining behavior is selective membrane-pore induction restricted to cells that display elevated HDM-2 at their surface, a marker of malignant transformation across several tumor types and largely absent in healthy tissue. That differential underpins the peptide's widespread application in tumor-selective membrane-disruption studies. Lyochem supplies PNC-27 as a lyophilized reference standard to a ≥99.0% HPLC purity specification. Spanning 32 residues with interleaved hydrophobic and cationic stretches, it sits toward the harder end of routine SPPS, so every released lot comes with its batch COA pairing peak-integration RP-HPLC and ESI-MS, with water-content determination and LC-MS/MS sequence confirmation available on request. One handling caveat: the penetratin domain drives adsorptive loss much as LL-37 does, so prepare sub-μg/mL working stocks in low-bind labware supplemented with BSA or Tween-20 carrier to keep effective concentration stable.
Applications & buyer fit
Longevity-class peptides — Epithalon, FoxO4-DRI, PNC-27 — ship to research labs studying telomere maintenance, senescence-clearance, and tumour-suppressor pathways. The chemistry is well-behaved but solution stability matters: Epithalon and FoxO4-DRI both prefer chelator-free water and storage at −20 °C protected from light. For chronic-exposure in vivo work, request bacterial-endotoxin testing on the specific lot at quote stage.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every lot has its own batch-specific CoA — HPLC purity and MS identity, plus any analytical scope agreed at quote stage — tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Research peptide; CAS is not consistently registered across suppliers. Sold for research use only.
Selected literature
Frequently asked questions
A fused two-domain sequence is long and repeat-prone, so intact mass alone is insufficient. RP-HPLC establishes purity and flags closely eluting deletion species that arise during synthesis of the longer construct. ESI-MS confirms the full-length mass, typically from a multiply-charged envelope that is deconvoluted to the neutral value. The decisive step, available on request, is LC-MS/MS sequencing: the b- and y-ion ladder demonstrates that the p53-derived binding domain and the penetratin transduction domain are joined in the labelled order, which distinguishes correct product from a truncated fusion of the same approximate mass. RP-HPLC purity and ESI-MS mass are reported on every batch COA, and the LC-MS/MS result is added when tested.
Handling should preserve the amphipathic, cell-penetrating character that the assay depends on. The penetratin domain makes the peptide surface-active, so it can adsorb to plasticware and bind filter membranes; low-binding tubes and minimal filtration reduce silent loss of the working concentration. Prepare a concentrated stock, aliquot for single use, and avoid repeated freeze-thaw, which is the dominant cause of measured-potency drift in peptides of this length. Confirm dissolved concentration against the certified net-peptide value rather than nominal weight before dosing, so cytotoxicity readouts are anchored to a verified input amount and remain comparable across lots.