We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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p53-HDM2-binding · penetratin-fused research peptide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying PNC-27 as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
PNC-27 is engineered as a bifunctional chimera: a p53-derived HDM-2-binding segment (residues 12-26) occupies the N-terminus, while the C-terminus carries a penetratin transduction domain drawn from Antennapedia-homeodomain residues 43-58. Functionally, the penetratin portion ferries the construct across the plasma membrane so the p53 element can contact intracellular HDM-2, the human MDM2 ortholog. Its defining behavior is selective membrane-pore induction restricted to cells that display elevated HDM-2 at their surface, a marker of malignant transformation across several tumor types and largely absent in healthy tissue. That differential underpins the peptide's widespread application in tumor-selective membrane-disruption studies. Lyochem supplies PNC-27 as a lyophilized reference standard at ≥99.0% HPLC. Spanning 32 residues with interleaved hydrophobic and cationic stretches, it sits toward the harder end of routine SPPS, so the release packet pairs peak-integration RP-HPLC and ESI-MS with water-content determination and optional LC-MS/MS sequence confirmation on request. One handling caveat: the penetratin domain drives adsorptive loss much as LL-37 does, so prepare sub-μg/mL working stocks in low-bind labware supplemented with BSA or Tween-20 carrier to keep effective concentration stable.
Applications & buyer fit
Longevity-class peptides — Epitalon, FoxO4-DRI, PNC-27 — ship to research labs studying telomere maintenance, senescence-clearance, and tumour-suppressor pathways. The chemistry is well-behaved but solution stability matters: Epitalon and FoxO4-DRI both prefer chelator-free water and storage at −20 °C protected from light. For chronic-exposure in vivo work, request bacterial-endotoxin testing on the specific lot at quote stage.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Research peptide; CAS is not consistently registered across suppliers. Sold for research use only.
Selected literature
Frequently asked questions
A fused two-domain sequence is long and repeat-prone, so intact mass alone is insufficient. RP-HPLC establishes purity and flags closely eluting deletion species that arise during synthesis of the longer construct. ESI-MS confirms the full-length mass, typically from a multiply-charged envelope that is deconvoluted to the neutral value. The decisive step is LC-MS/MS sequencing: the b- and y-ion ladder demonstrates that the p53-derived binding domain and the penetratin transduction domain are joined in the labelled order, which distinguishes correct product from a truncated fusion of the same approximate mass. We report all three results per lot.
Handling should preserve the amphipathic, cell-penetrating character that the assay depends on. The penetratin domain makes the peptide surface-active, so it can adsorb to plasticware and bind filter membranes; low-binding tubes and minimal filtration reduce silent loss of the working concentration. Prepare a concentrated stock, aliquot for single use, and avoid repeated freeze-thaw, which is the dominant cause of measured-potency drift in peptides of this length. Confirm dissolved concentration against the certified net-peptide value rather than nominal weight before dosing, so cytotoxicity readouts are anchored to a verified input amount and remain comparable across lots.