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0.9% isotonic sodium-chloride reconstitution diluent
Overview
0.9% Sodium Chloride Injection (isotonic saline) is the standard neutral-pH, physiological-osmolarity reconstitution diluent for peptides that are soluble and stable at neutral pH. The 0.9% NaCl concentration gives a solution isotonic with blood plasma (≈ 300 mOsm/L), which minimizes osmotic stress when reconstituted material is introduced into cell-culture systems or in vivo models. It is the workhorse diluent for the majority of synthetic-peptide reconstitution workflows without aggregation tendency — in contrast to proteins like IGF-1 LR3 that require the acidic-pH acetic-acid diluent instead. It is supplied as a characterized ancillary diluent rather than a research-target molecule. Lyochem supplies 0.9% Saline in sterile vial formats prepared from pharmaceutical-grade sodium chloride and Water for Injection. The release packet documents sterility, bacterial endotoxin (LAL), osmolality, and pH per pharmacopeia standards; the diluent is supplied to the USP monograph by default, with EP / BP / JP equivalents available on request for buyers whose destination market requires a specific monograph reference.
Applications & buyer fit
Solvents and ancillaries are the bench-support consumables a lab pairs with a reference standard once it leaves the freezer — bacteriostatic and sterile water, isotonic saline, 0.6% acetic-acid water, and glacial acetic acid for in-house diluent prep. These are diluents and process auxiliaries rather than research targets, so the documentation is diluent / solvent-grade (component identity, sterility, endotoxin, pH / osmolality, pharmacopeia monograph) rather than the peptide HPLC + MS + sequence packet. Diluent selection follows the peptide's stability profile: acidic-pH-requiring proteins use the acetic-acid water; preservative-incompatible peptides use sterile water; preservative-compatible peptides for multi-use vials use BAC water.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Core Facilities & Instrumentation Cores
Shared instrumentation cores, mass-spec facilities, and departmental analytical labs holding reference standards for cross-PI method validation and reagent qualification.
Contract Research Organisations (CROs)
CROs running preclinical and translational studies on behalf of academic, biotech, or pharma sponsors who need traceable reference material across multi-site campaigns.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Isotonic reconstitution diluent; supplied for Research Use Only. Verify sterility, pyrogen (LAL), osmolality, and injectable-grade compliance with the destination market's requirements before use.
Frequently asked questions
The 0.9% NaCl concentration (≈ 300 mOsm/L) matches the osmolality of blood plasma and extracellular fluid, so it drives no net water movement across cell membranes — avoiding the hemolysis of hypotonic preparations or the cell-shrinkage of hypertonic ones. For peptide reconstitution this produces working solutions physiologically compatible with both in vivo administration and most cell-culture buffers. Peptides requiring acidic pH use the 0.6% acetic-acid diluent instead; single-use preservative-free preparations use SWFI.
0.9% Sodium Chloride Injection is monographed in USP, EP, BP, and JP. The specifications cover identity (sodium and chloride content), sterility, bacterial endotoxin (LAL), pH, particulate matter, and osmolality. Lyochem supplies to the USP monograph by default; buyers in EU, UK, or APAC markets needing a local-pharmacopeia spec should specify at quote stage so the documentation references the appropriate monograph.
Proteins and peptides that aggregate or precipitate at neutral pH — IGF-1 LR3 and unmodified MGF are the common examples — should use the 0.6% acetic-acid diluent rather than saline. The peptide's recommended diluent is noted on its COA; saline is the default for the large majority of synthetic peptides that are soluble and stable at neutral pH.
Related peptides
0.9% benzyl-alcohol bacteriostatic reconstitution diluent (multi-use)
Preservative-free sterile-water reconstitution diluent (single-use)