We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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NNMT inhibitor (5-Amino-1-methylquinolinium iodide)
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying 5-Amino-1MQ as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
5-Amino-1MQ (5-amino-1-methylquinolinium iodide) is a small-molecule inhibitor that selectively targets nicotinamide N-methyltransferase (NNMT), the enzyme using SAM to methylate nicotinamide. Blocking NNMT redirects nicotinamide away from the methylated dead-end product and back into NAD+ salvage, raising intracellular NAD+ most noticeably where NNMT is highly expressed. It has been profiled in adipocyte metabolism, energy-expenditure, and obesity models, on the premise that NNMT inhibition targets a metabolic-pathway contributor to the NAD+ decline associated with aging and obesity. Lyochem supplies 5-Amino-1MQ in its iodide-salt form as a reference standard at ≥99.0% HPLC. Being a small molecule rather than a peptide, its release relies on RP-HPLC purity, UV-Vis identity, and residual-solvent screening by GC-MS or LC-MS. Fill sizes of 5, 10, and 50 mg cover most bench workflows.
Applications & buyer fit
Mitochondrial-targeted peptides — MOTS-c, SS-31 (Elamipretide) — and the mitochondrial-targeting small-molecule reagents (NAD+, AICAR, 5-Amino-1MQ, SLU-PP-332) ship to research labs studying OXPHOS, ROS biology, sirtuin-mediated deacetylation, and mitochondrial dysfunction in disease models. The lipophilic / cationic character that drives mitochondrial accumulation also makes some peptides oxidation-prone in solution; working stocks should be prepared fresh or held at −80 °C when the workflow permits.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Salt form (iodide vs. other) may vary across suppliers; review the batch COA.
Frequently asked questions
For a quinolinium salt, a two-part release panel is appropriate. RP-HPLC with UV detection resolves the cation from synthesis-related impurities and gives the chemical-purity figure, while ESI-MS in positive-ion mode confirms the intact cation mass since the iodide dissociates in solution and is not seen on the cation. The counter-ion itself is quantified separately, typically by ion chromatography or an argentometric assay, so the reported salt stoichiometry on the COA reflects measured iodide content rather than an assumption. Karl Fischer water content rounds out the mass-balance picture for accurate molar dosing in NNMT-inhibition assays.
Treat the labelled purity and measured water content as inputs to a corrected weighing so nominal molarity of the DMSO stock is accurate. Because the cation can slowly hydrolyze at neutral pH, prepare aqueous working dilutions immediately before use and keep the concentrated DMSO stock cold to preserve integrity between experiments. For a reference-standard role, retain a small aliquot of the qualified lot, re-verify identity by ESI-MS if a stock has aged, and record the COA lot number in the methods section so downstream NNMT readouts remain traceable to a single characterized batch of material.
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