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EPO-derived 11-mer · cytoprotective research peptide
Research documentation
Ask sales for the ARA 290 (Cibinetide) batch COA and the analytical evidence needed to qualify the material for your study. Confirm the available purity and identity records, lot continuity, handling information, and method requirements before ordering.
Overview
ARA-290 (Cibinetide; pHBSP) is an 11-residue synthetic peptide modeled on helix B of erythropoietin (EPO). Its design intent was to preserve EPO's cytoprotective and anti-inflammatory signaling, which runs through the heteromeric Innate Repair Receptor formed by the EPO receptor together with CD131, while shedding the erythropoietic activity that EPO exerts via the classical EPO-receptor homodimer. Separating tissue protection from red-cell stimulation is the defining pharmacological feature of the molecule and the rationale for its clinical investigation across diabetic neuropathy, corneal nerve-damage research, and the small-fiber neuropathy associated with sarcoidosis. Lyochem supplies ARA-290 acetate as a lyophilized reference standard to a ≥99.0% HPLC purity specification. The 11-residue chain synthesizes reliably by SPPS, and every released lot comes with its batch COA recording integrated-peak HPLC and ESI-MS; water content and counter-ion data and LC-MS/MS sequence verification are available on request. Two fills, 10 mg and 16 mg, cover most work; the 16 mg size maps directly onto certain clinical protocols for lot-to-protocol correspondence. For in vivo neuropathy or inflammation studies we recommend adding endotoxin (LAL) and microbial-limits testing, since those readouts can be confounded by endotoxin-driven inflammatory pathways. Supplied for research use only.
Applications & buyer fit
Buyers in this category are research labs studying immune-modulation, cytokine signalling, and antimicrobial activity. The defining QC requirement is bacterial-endotoxin control: many downstream assays (NF-κB reporters, macrophage-activation panels, neutrophil-priming readouts) are themselves activated by endotoxin contamination, so a clean LAL on the specific lot is a precondition rather than a nice-to-have. LL-37 and related cationic antimicrobial peptides additionally benefit from low-bind plasticware during dilution.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every lot has its own batch-specific CoA — HPLC purity and MS identity, plus any analytical scope agreed at quote stage — tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Sold for Research Use Only under the receiving laboratory's institutional and jurisdictional regulations. Not a finished dosage form, not labelled for human administration, not for in-vitro diagnostic use. For NF-κB reporter, macrophage, neutrophil, and any other endotoxin-sensitive assay, request LAL testing on the specific lot as a precondition.
Selected literature
Frequently asked questions
ARA-290 is a short linear peptide derived from helix B of EPO, which makes it a straightforward target for a standard peptide-identity panel. RP-HPLC with UV detection resolves the main peak from deletion and truncation impurities and reports chemical purity, while ESI-MS confirms the intact mass. For unambiguous identity, LC-MS/MS sequence verification covering the b- and y-ion series confirms the residue order rather than mass alone, which is important given how many short peptides sit at similar masses. Karl Fischer water content and net peptide content complete the picture so the qualified lot can serve as a dependable comparison standard.
Reconstitute in a compatible aqueous vehicle, aliquot for single use, and hold cold to limit freeze-thaw exposure, since repeated cycling and prolonged solution storage are the usual routes to aggregation or degradation in short peptides. Use the labelled net peptide content and measured water to weigh a corrected amount so stated molarity is accurate for Innate Repair Receptor studies. If a stock has aged before a key experiment, re-checking identity and purity by RP-HPLC and ESI-MS is inexpensive insurance, and recording the COA lot keeps cytoprotection readouts traceable to a single characterized batch of material.
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