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Off-spec? Free replacement or full refund.
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Reference standard · Research use only
Cobalamin reference standard · cyanocobalamin (68-19-9) or methylcobalamin (13422-55-4) form
Price, MOQ and lead time come in your written quote — usually within 24 hours. No account needed.
Compound facts
| Name | B12 |
|---|---|
| CAS number | 68-19-9 (Cyanocobalamin) / 13422-55-4 (Methylcobalamin) |
| PubChem CID | 5311498 |
| Molecular formula | C63H88CoN14O14P |
| Molecular weight | 1355.4 g/mol |
| Purity specification (HPLC) | ≥ 99.0% |
| On every batch COA | RP-HPLC purity · ESI-MS identity · Bacterial endotoxin · Fill amount (content per vial) |
| On request | LC-MS/MS sequence confirmation · Net peptide content · Water content (Karl Fischer) · Counter-ion content (e.g. acetate, TFA) · Microbial limits |
| Storage | Refrigerated 2–8°C, protect from light |
| Strengths per vial | 1 mg/ml · 10 ml |
| Pack | 10 vials per kit |
| MOQ | On request |
| Lead time | 7–14 days |
| Use | Research use only — not for human or veterinary use |
Identity data from the Lyochem product catalogue, checked against PubChem and registry records. The batch COA of your lot is the record for that lot's results. How to verify a COA
Lot documentation
Values belong to the lot. Ask for the COA of the offered lot before you order.
Independent test fails? Full refund. How it works
Overview
Vitamin B-12 (cobalamin) is a cobalt-containing organometallic vitamin and an essential cofactor for methionine synthase and methylmalonyl-CoA mutase. It exists in four biologically relevant forms distinguished by the ligand at the cobalt centre — cyanocobalamin (-CN, the synthetic / supplemental form), methylcobalamin (-CH₃, the cytoplasmic methyl-donor form), hydroxocobalamin (-OH), and adenosylcobalamin (5'-deoxyadenosyl, the mitochondrial form). The forms are not interchangeable as standards, so the specific form is the central identity question. B-12 is referenced here as a characterized standard, not for any clinical outcome. Lyochem supplies B-12 as a reference standard in both cyanocobalamin (CAS 68-19-9) and methylcobalamin (CAS 13422-55-4) forms; specify the form at order placement, with hydroxo- and adenosyl- forms available on request. Every released lot comes with its batch COA reporting chemical purity by HPLC and mass-spec identity; the exact form and salt and the water content are listed when included in the agreed analytical scope. All cobalamin forms are photosensitive and oxidation-sensitive, so storage is refrigerated and light-protected. Confirm the form by COA / label before use, since the forms differ in their applications. This is reference-grade research material, distinct from compounding-scale API supply.
Applications & buyer fit
Other research peptides in this category — Melanotan I, Melanotan II, PT-141, ACE-031, Adipotide / FTPP, EPO, HMG — ship to research labs studying topics outside the GH / cognitive / immune / mitochondrial / repair / longevity / Khavinson clusters. Each peptide has its own analytical-packet emphasis (e.g. glycoprotein bioassay in IU for HMG; cyclised-form confirmation for oxytocin) noted in the per-product CoA scope.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Biotech R&D Groups
Preclinical biotech and pharmaceutical discovery teams sourcing characterized peptides for receptor-pharmacology, screening, and method-development campaigns.
Every lot has its own batch-specific CoA — RP-HPLC purity, ESI-MS identity, bacterial endotoxin and fill amount, plus any analytical scope agreed at quote stage — tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Other formats
B12 is also kept as a solution in nasal spray bottles.
| Contents | 0.5 mg/mL |
|---|---|
| Fill volume | 10 mL or 20 mL |
| Minimum | 10 bottles per content and volume |
| Stock | Held in the United States, shipped within the US |
Regulatory note
Specify the exact B-12 form (cyano / methyl / hydroxo / adenosyl) at order placement; the forms are not interchangeable across applications. Confirm form and salt by batch COA / label before use. Lyochem supplies B-12 strictly for Research Use Only; buyers are responsible for verifying ingredient eligibility in their destination market.
Frequently asked questions
It depends on the reference need. Cyanocobalamin (CAS 68-19-9) is the more shelf-stable form and the common supplemental and injectable reference; it requires intracellular conversion to the active forms. Methylcobalamin (CAS 13422-55-4) is the directly active cytoplasmic methyl-donor form, preferred where the work needs the active species without the conversion step. Hydroxo- and adenosyl- forms are available on request; specify the form at order placement since the forms are not interchangeable as standards.
All four cobalamin forms are photosensitive — light initiates photochemical degradation of the corrin ring — and the cobalt centre is oxidation-sensitive under unprotected conditions. Refrigerated (2–8 °C) storage with light protection (amber or opaque packaging) keeps the 1 mg/mL solution within specification over its shelf life; keep it in the original container until use.
The batch COA reports chemical purity by HPLC and mass-spec identity; the exact form and salt and the water content are listed when tested. Because the forms are not interchangeable across applications, a lab should confirm the form on the COA or label before use rather than relying on the catalog name alone.
Yes: 0.5 mg/mL, in 10 mL or 20 mL bottles, minimum 10 bottles per content and volume. This format is held in the United States and shipped within the US; contact sales for availability and a quotation.
Send any vial from your order to an independent laboratory of your choice. If the report shows the product does not meet the specification on its COA — purity below the stated minimum, or an identity that does not match — share the report with our sales team and we refund that product in full.
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