We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
Reading a Peptide HPLC Trace — A 5-Minute Field Guide for Bench Scientists. Read our briefing →
Reading a peptide HPLC trace — a field guide. Read →
On reading HPLC traces. Read →
Delta sleep-inducing peptide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying DSIP as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
A nonapeptide of sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, DSIP (Delta Sleep-Inducing Peptide) was first recovered from the cerebral venous blood of rabbits during electrical stimulation of sleep-promoting brain regions. Since the 1970s it has been examined for a putative part in slow-wave (delta) sleep regulation, in stress-response modulation, and in neuroendocrine effects on cortisol and GH release. Its receptor target has never been pinned down, and that mechanistic obscurity, greater than for most other cognitive-research peptides, is much of what sustains ongoing interest in the molecule. Lyochem supplies DSIP as a lyophilized reference standard at ≥99.0% HPLC. Routine characterization comprises integrated-peak RP-HPLC, ESI-MS identity, water content, and counter-ion, and standard fills of 2-15 mg suit most workflows. One handling caveat deserves emphasis: DSIP is markedly more hygroscopic than typical short peptides, so vials are best reconstituted soon after opening and any leftover powder kept sealed under nitrogen or dry argon. Reconstituted material should be split into single-use aliquots and stored at -20 °C with disciplined freeze-thaw control.
Applications & buyer fit
Cognitive and neuropeptide buyers are predominantly research labs running in vivo rodent studies. The dominant administration route in the published literature is intranasal — Semax, Selank, DSIP, Pinealon — because these peptides are not meaningfully blood-brain-barrier permeable when delivered systemically. For in vivo workflows, endotoxin and microbial-limit testing is recommended at the CoA stage so the bioassay readout is not confounded by contamination unrelated to the test article.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Sold for Research Use Only under the receiving laboratory's institutional and jurisdictional regulations. Not a finished dosage form and not labelled for human administration. In vivo research workflows should request endotoxin and microbial-limit testing on the specific lot so the bioassay readout is not confounded by contamination.
Selected literature
Frequently asked questions
Pharmacological uncertainty about the binding site has no bearing on chemical characterization, which relies on the known nonapeptide structure. ESI-MS or LC-MS establishes the intact molecular mass, and MS/MS fragmentation can read the residue order to confirm the sequence directly. RP-HPLC then reports chromatographic purity and separates any deletion or hydrolysis products. Together these fix identity and purity independently of any functional assay, which is appropriate for a molecule still used as an investigational reference tool. The certificate should carry the observed mass, the purity method, and the salt form for accurate stock preparation.
The sequence is unusually polar, rich in Asp, Glu, Ser, and Gly with few hydrophobic residues, so the lyophilizate takes up ambient moisture quickly. That absorbed water inflates the vial mass and, if uncorrected, causes a reconstituted stock to fall a few percent below the intended concentration. Reliable work therefore depends on a residual-water figure, for example by Karl Fischer, determined close to fill and reported on the certificate, together with the counter-ion form. In the laboratory, keep open-vial time short, reconstitute promptly, and store partially used vials under dry nitrogen or argon to limit further uptake.