We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
Reading a Peptide HPLC Trace — A 5-Minute Field Guide for Bench Scientists. Read our briefing →
Reading a peptide HPLC trace — a field guide. Read →
On reading HPLC traces. Read →
ACTH 4-10 analog · cognitive research peptide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying Semax as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
A synthetic heptapeptide built on the ACTH(4-10) core sequence Met-Glu-His-Phe-Pro-Gly-Pro, Semax carries a Pro-Gly-Pro tail at the C-terminus that shields it against peptidase attack and extends its functional persistence well beyond the parent ACTH fragment. Investigational interest centers on modulation of BDNF expression together with neuroprotective, working-memory, and attentional readouts. First created during the 1980s at Moscow's Institute of Molecular Genetics, it holds prescription status in Russia for cerebrovascular and cognitive indications while remaining unapproved across the US and EU. Lyochem supplies Semax acetate as a lyophilized reference standard at ≥99.0% HPLC, released against RP-HPLC purity, ESI-MS identity, water content, and acetate counter-ion determination. Nearly all reported studies administer it intranasally: systemic dosing does not deliver meaningful amounts across the blood-brain barrier, whereas the nasal route exploits direct uptake through olfactory and respiratory epithelium. When reconstituting for intranasal work, isotonic vehicles such as saline or PBS are preferable to plain water, and preservatives like benzalkonium chloride should be avoided because they interact with short peptides. Fill sizes of 2-15 mg span routine aliquoting through larger dispensing needs.
Applications & buyer fit
Cognitive and neuropeptide buyers are predominantly research labs running in vivo rodent studies. The dominant administration route in the published literature is intranasal — Semax, Selank, DSIP, Pinealon — because these peptides are not meaningfully blood-brain-barrier permeable when delivered systemically. For in vivo workflows, endotoxin and microbial-limit testing is recommended at the CoA stage so the bioassay readout is not confounded by contamination unrelated to the test article.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Biotech R&D Groups
Preclinical biotech and pharmaceutical discovery teams sourcing characterized peptides for receptor-pharmacology, screening, and method-development campaigns.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
In Russia this is an approved prescription medication indicated for cerebrovascular and cognitive conditions; it remains unapproved across the US and EU. Lyochem supplies Semax strictly for Research Use Only, and buyers bear responsibility for verifying that the ingredient is eligible in their destination market.
Selected literature
Frequently asked questions
Semax is a defined heptapeptide, so the confirmation set centers on chromatography and mass. RP-HPLC on a C18 column provides the purity figure and separates deletion or unacetylated by-products from the main peak. ESI-MS confirms the intact mass against the theoretical value for the sequence, resolving from a multiply-charged envelope where needed. For unambiguous sequence assignment the short chain is amenable to LC-MS/MS residue-ladder confirmation. Each of these measured outputs is transcribed onto the batch certificate, so a lab can cite the actual purity and mass for its lot in method documentation rather than a generic catalog claim.
The acetylation is the analytical marker. N-terminal acetylation adds a fixed mass increment, so ESI-MS reads the acetylated variant heavier than unmodified Semax by that increment, and the difference is unambiguous for a peptide this size. The added acetyl group also raises hydrophobicity, shifting RP-HPLC retention under a fixed gradient, so a side-by-side injection against a known reference separates the two forms. We state which form a given SKU represents and report its measured mass and retention on the certificate, so a receiving lab can confirm it holds the unmodified standard or the acetylated analog before use in an assay.