We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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Gonadotropin glycoprotein hormone · heavily regulated
Lyochem analytical fit
This Lyochem page should win analytical and reference-lot searches for HCG rather than regulated procurement searches. The buyer is a lab, CRO, or method team that needs lot identity, assay comparison, and documented research-use qualification before adding the material to a method or validation file.
Overview
Human Chorionic Gonadotropin (HCG) is a heterodimeric glycoprotein hormone secreted natively by placental syncytiotrophoblast cells in pregnancy. It pairs a 92-amino-acid alpha-subunit — common to FSH, LH, and TSH — non-covalently with a 145-amino-acid HCG-specific beta-subunit, both extensively glycosylated, and it activates the shared LH/HCG receptor to reproduce LH-like signaling. Prescription approvals cover ovulation induction, male hypogonadotropic hypogonadism, and cryptorchidism in pediatric patients. Bulk material derives either from purified pregnancy urine (the classical route) or from recombinant cell culture; the two sources differ in glycan structure and each calls for source-specific identity confirmation. Lyochem releases HCG only to appropriately licensed buyers. Eight standard fills bridge the two dosing conventions — IU-based (1000–10000 IU) and mass-based (1–10 mg) — to fit research and academic workflows. Because heterodimer integrity and glycosylation govern potency in ways chromatographic purity cannot report, the reference-standard release centers on biological activity expressed as IU per mg by bioassay rather than chemical purity alone.
Applications & buyer fit
Biotech R&D Groups
Preclinical biotech and pharmaceutical discovery teams sourcing characterized peptides for receptor-pharmacology, screening, and method-development campaigns.
Contract Research Organisations (CROs)
CROs running preclinical and translational studies on behalf of academic, biotech, or pharma sponsors who need traceable reference material across multi-site campaigns.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
A compliance review is required before any order proceeds. This heterodimeric glycoprotein hormone is highly regulated, and we supply it exclusively to qualified buyers who hold appropriate licensing in their jurisdiction.
Selected literature
Frequently asked questions
HCG is a heterodimeric glycoprotein hormone, so its labeled activity is defined by an International Unit bioassay calibrated against a reference standard, not by protein mass, because glycosylation strongly influences biological activity. Physicochemical identity therefore combines complementary methods: SDS-PAGE and SEC for the alpha/beta subunit size and aggregation, LC-MS or peptide mapping for backbone confirmation, and isoelectric focusing or capillary electrophoresis to profile the glycoform charge distribution. The COA carries the batch-specific IU-per-mg from bioassay as the operative potency figure. Buyers qualifying a reference lot should treat the bioassay value, tied to source (urinary vs recombinant), as the governing specification.
Urinary-derived and recombinant HCG share the same protein backbone but carry different glycosylation, which is why their specific activities differ substantially per unit mass and why the mass-to-IU conversion is source-dependent. For analytical qualification this means the glycoform profile (by isoelectric focusing or charge-based electrophoresis) is part of the identity picture, and the bioassay-derived IU/mg must be read in the context of the declared source. Confirm on the COA which source the batch represents, since applying a recombinant conversion factor to urinary material, or vice versa, would misstate the effective units in any concentration-controlled experiment.
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