We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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IGF-1 Ec splice-variant peptide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying MGF (Mechano Growth Factor) as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
MGF (Mechano Growth Factor) is the 24-residue C-terminal E-peptide encoded by the IGF-1 Ec transcript, an alternatively spliced product of the IGF-1 gene whose E-domain extension differs from that of the predominant IGF-1Ea isoform. Skeletal muscle subjected to mechanical loading and exercise stress upregulates the Ec variant preferentially, and this load-responsive expression is the origin of the name. Rather than signaling through the canonical IGF-1 receptor, the isolated E-peptide is proposed to engage a separate target and to promote satellite-cell proliferation in models of muscle repair. What Lyochem supplies is the synthetic C-terminal E-peptide on its own, not the intact IGF-1 Ec protein, delivered as a lyophilized reference standard at ≥99.0% HPLC in a 2 mg vial. Because the native molecule persists only minutes in plasma, PEGylated MGF is the form typically chosen for in vivo work, whereas this unmodified standard suits cell-culture investigations where rapid clearance is irrelevant. Purity is assessed by RP-HPLC and mass confirmed by ESI-MS; given that the sequence is shared with a segment of IGF-1, LC-MS/MS peptide mapping (rather than intact-mass alone) is advised when qualifying a new lot, and water content and counter-ion are reported on the COA.
Applications & buyer fit
GH-axis peptides ship to research labs studying somatotropic-pathway pharmacology, IGF-axis signalling, and pulse vs. sustained-elevation GH biology. Buyers qualifying a new source typically request sequence verification on the first lot, the counter-ion form (acetate by default), and stability data at −20 °C. Blends — the CJC-1295 + Ipamorelin co-formulated lot is the canonical example — are co-lyophilised rather than solution-mixed so the ratio is locked at the lyophilisation step.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Across suppliers, the CAS number for unmodified MGF is not consistently standardized; verify the sequence together with PEG status via the batch COA (unmodified MGF should carry no PEG, if any is present).
Selected literature
Frequently asked questions
MGF is a 24-residue C-terminal peptide arising from the IGF-1Ec splice variant, whereas mature IGF-1 is a 70-residue folded growth factor, so intact-mass measurement by ESI-MS or LC-MS separates them unambiguously by size. RP-HPLC purity confirms MGF as a single dominant species. The defining structural element is the E-domain sequence absent from the common IGF-1Ea form, so an analyst verifying identity should confirm the mass corresponds to the short E-peptide construct rather than any IGF-1 fragment, since only that sequence supports the E-peptide-specific signaling the material is intended to probe.
Unmodified MGF has a very short functional lifetime, appropriate to its role as a local autocrine signal but limiting for the analyst. In practice this means reconstituting immediately before use, keeping reconstituted material cold and briefly held, and designing short-duration cell-culture or biochemical assays where minutes-scale stability suffices. Workflows needing sustained exposure use the PEGylated PEG-MGF form instead. For reference-standard identity confirmation by HPLC and mass spec the unmodified peptide is fine, since those measurements are made on freshly dissolved material before appreciable degradation occurs.
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