We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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PEGylated Mechano Growth Factor
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying PEG MGF as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
PEG-MGF pairs MGF, the IGF-1 splice variant known as Mechano Growth Factor, with a covalently attached polyethylene-glycol chain, the objective being to overcome the minutes-scale half-life of the unmodified peptide. The chemistry mirrors clinically established PEG-protein conjugates such as PEG-IFN and PEG-G-CSF: bolting a PEG polymer onto reactive residues enlarges the hydrodynamic radius, slows glomerular clearance, and sterically blocks proteases. Critically, sourcing is inconsistent across the market, with polymer sizes ranging from 5 kDa to 40 kDa and conjugation performed either site-specifically at a single residue or randomly across multiple sites. Lyochem supplies PEG-MGF as a lyophilized reference standard at ≥99.0% HPLC. Since polymer mass and attachment position dictate pharmacokinetics, each batch COA states the PEG molecular weight, the conjugation mode (site-specific versus random), and the PEG-to-peptide stoichiometry explicitly. That documentation matters: two lots both labeled 'PEG-MGF' can diverge by 5-10× in functional half-life purely on PEG architecture, so cross-supplier comparisons should be normalized against these conjugate parameters rather than judged on peptide loading alone.
Applications & buyer fit
Repair peptides — BPC-157, TB-500, B7-33, PEG-MGF — ship primarily to research labs studying tissue-repair, gastrointestinal-mucosa, tendon-ligament, and vascular-endothelial models. The synthesis itself is reliable, but analytical confirmation is where suppliers differ: buyers qualifying a new source should request sequence verification by tandem MS on the first lot and compare against the labelled sequence directly.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Because this is a PEG conjugate whose architecture differs from one supplier to the next, pharmacokinetic comparability across supplier sources cannot be assumed. On each batch COA, confirm the PEG molecular weight, the attachment site, and the PEG-to-peptide ratio.
Selected literature
Frequently asked questions
Because the conjugate is a peptide plus a polymer, identity work has two halves. RP-HPLC resolves the PEGylated species from residual free MGF and from over- or under-conjugated by-products, since the polymer shifts retention markedly. ESI-MS or MALDI then reports a broadened mass envelope rather than a single sharp peak, because the polydisperse PEG contributes a distribution of masses; the centroid should track the theoretical peptide mass plus the nominal polymer mass. We reproduce the chromatogram and the observed mass centroid on the COA so a receiving lab can verify conjugation occurred and estimate the peptide-to-polymer stoichiometry directly.
Polydispersity is the core complication. A defined peptide gives one exact monoisotopic mass, so a reference lot is straightforward to certify. The attached polymer instead spans a range of chain lengths, so mass spectrometry returns an envelope and no single formula fully describes the material; positional isomers from non-site-specific attachment add further heterogeneity that co-migrates. For assay use this means potency should be normalized to verified peptide content, not to gross weight, and the certificate should state which attachment chemistry and nominal polymer size the lot represents. We document these parameters per batch so results remain traceable across re-orders.
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