We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
Reading a Peptide HPLC Trace — A 5-Minute Field Guide for Bench Scientists. Read our briefing →
Reading a peptide HPLC trace — a field guide. Read →
On reading HPLC traces. Read →
Nicotinamide adenine dinucleotide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying NAD+ as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
NAD+ (Nicotinamide Adenine Dinucleotide, oxidized form) is a redox coenzyme that also serves as substrate for sirtuin deacetylation, PARP-driven DNA-damage response, and CD38 calcium signaling. Its tissue levels fall with age across mammals, and reversing that decline is the organizing premise of much longevity research — hence the sustained study of precursors such as NMN and NR alongside direct NAD+ supplementation. It is not a peptide; Lyochem lists it because it is routinely co-administered with peptide research products and sits at the center of cellular-energy-metabolism workflows. Lyochem supplies NAD+ at ≥99.0% HPLC in lyophilized form. The release panel pairs RP-HPLC purity with UV-Vis absorbance read at the diagnostic 260 nm adenine band (used to confirm identity and estimate content), plus residual-solvent profiling. The 100, 500, and 1000 mg fills reflect the gram-scale, stoichiometric consumption typical of biochemical-assay and in vivo research — far above the μg-mg quantities of peptide work. Store lyophilized material at -20 °C shielded from light; reconstituted NAD+ is moisture- and oxidation-sensitive and should be used without delay.
Applications & buyer fit
Mitochondrial-targeted peptides — MOTS-c, SS-31 (Elamipretide) — and the mitochondrial-targeting small-molecule reagents (NAD+, AICAR, 5-Amino-1MQ, SLU-PP-332) ship to research labs studying OXPHOS, ROS biology, sirtuin-mediated deacetylation, and mitochondrial dysfunction in disease models. The lipophilic / cationic character that drives mitochondrial accumulation also makes some peptides oxidation-prone in solution; working stocks should be prepared fresh or held at −80 °C when the workflow permits.
Biotech R&D Groups
Preclinical biotech and pharmaceutical discovery teams sourcing characterized peptides for receptor-pharmacology, screening, and method-development campaigns.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Sold for Research Use Only under the receiving laboratory's institutional and jurisdictional regulations. Not a finished dosage form and not labelled for human administration. Working stocks of cationic mitochondrial peptides (SS-31 in particular) should be prepared fresh or held at −80 °C when the workflow permits.
Selected literature
Frequently asked questions
NAD+ is a dinucleotide coenzyme, nicotinamide mononucleotide joined to adenosine monophosphate through a pyrophosphate bridge, not a peptide, so it carries no sequence to verify. Identity and purity rely on HPLC together with UV-Vis at 260 nm, the adenine absorbance, rather than the HPLC-plus-mass-spec-plus-sequence packet used for peptides. An analyst should read the certificate for a chromatographic purity figure and the expected 260 nm spectral signature, and should note that the relevant impurities are nucleotide breakdown products, so purity is judged against a different degradation profile than a peptide's.
In solution NAD+ is more prone to moisture-driven hydrolysis than a lyophilized peptide, so reconstituted material should be prepared fresh and used promptly rather than kept as a standing working stock that accumulates hydrolysis products. Because many assays consume it stoichiometrically at millimolar concentrations, sizable amounts are dissolved per replicate, making fresh preparation both a stability and an accuracy safeguard. Unused solid is best kept sealed under nitrogen at -20 C to limit water uptake, preserving the integrity an analyst depends on when the 260 nm reading is used to set concentrations.