We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
Reading a Peptide HPLC Trace — A 5-Minute Field Guide for Bench Scientists. Read our briefing →
Reading a peptide HPLC trace — a field guide. Read →
On reading HPLC traces. Read →
Mitochondrial-targeted peptide
Lyochem primary owner
This Lyochem page is the primary SEO owner for research labs, CROs, and method-development teams qualifying SS-31 (Elamipretide) as a documented research-standard lot. The page should answer whether the buyer can review HPLC purity, identity confirmation, lot continuity, stability handling, and assay-fit documentation before ordering.
Overview
A synthetic cationic-aromatic tetrapeptide, SS-31 (Elamipretide, Bendavia, MTP-131) carries the sequence D-Arg-Dmt-Lys-Phe-NH₂, in which Dmt denotes 2',6'-dimethyl-L-tyrosine. Its alternating charge-and-aromatic motif — the defining feature of the Szeto-Schiller cell-permeable, mitochondria-targeted class — lets the peptide accumulate passively across the inner mitochondrial membrane along the negative membrane potential. There it associates with cardiolipin and shields that lipid from peroxidation; preserving cardiolipin in turn stabilizes cristae architecture and OXPHOS supercomplex function. This mechanism underlies the molecule's clinical-development work in mitochondrial dysfunction disorders such as Barth syndrome and primary mitochondrial myopathies, and in acute-injury settings including ischemia-reperfusion injury and contrast-induced nephropathy. Lyochem supplies SS-31 acetate as a lyophilized reference standard at ≥99.0% HPLC purity. Because the non-natural Dmt residue is built from specialized Fmoc-protected reagents and shifts the mass +28 Da versus the natural-Tyr analog, ESI-MS confirmation of that modified mass serves as the primary identity check on every batch. We recommend LC-MS/MS sequence verification at first-time supplier qualification, since Dmt-substituted peptides can co-elute with biologically inactive unmodified analogs. Counter-ion and residual-water content are reported alongside chromatographic purity, and three fill sizes (5, 10, 50 mg) span standard research and compounding needs.
Applications & buyer fit
Mitochondrial-targeted peptides — MOTS-c, SS-31 (Elamipretide) — and the mitochondrial-targeting small-molecule reagents (NAD+, AICAR, 5-Amino-1MQ, SLU-PP-332) ship to research labs studying OXPHOS, ROS biology, sirtuin-mediated deacetylation, and mitochondrial dysfunction in disease models. The lipophilic / cationic character that drives mitochondrial accumulation also makes some peptides oxidation-prone in solution; working stocks should be prepared fresh or held at −80 °C when the workflow permits.
Academic Laboratories
Universities, medical schools, and government research institutes qualifying a reference standard for a method-development or in vivo workflow.
Biotech R&D Groups
Preclinical biotech and pharmaceutical discovery teams sourcing characterized peptides for receptor-pharmacology, screening, and method-development campaigns.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
Sold for Research Use Only under the receiving laboratory's institutional and jurisdictional regulations. Not a finished dosage form and not labelled for human administration. Working stocks of cationic mitochondrial peptides (SS-31 in particular) should be prepared fresh or held at −80 °C when the workflow permits.
Selected literature
Frequently asked questions
ESI-MS confirms the intact molecular mass, but for a tetrapeptide carrying Dmt and two basic residues the mass value alone cannot rule out a positional isomer or a residue swap of equal mass. The release packet therefore pairs ESI-MS with an RP-HPLC main-peak integration and, at first-time qualification, LC-MS/MS fragment coverage that reads the residue order directly. Because the natural-Tyr analog differs by only the two Dmt methyls, the mass offset from that analog is itself a useful identity marker, distinguishing correctly synthesized material from a truncated tyrosine variant.
Because SS-31 carries a net +3 charge, the lyophilized solid is isolated as a salt, so the certified counter-ion identity and the residual water measured by Karl Fischer both sit between the weighed mass and the true peptide content. A vial reported as net peptide may be 15-25% salt and water by mass, and dosing off the gross fill would overstate the concentration delivered to a cardiolipin-binding or mitochondrial-uptake assay. Ask that the COA state peptide content net of counter-ion and water so molar working concentrations trace back to actual peptide.
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