We report net peptide content, not just purity — so the milligrams you dose aren't padded with water and counter-ion. RP-HPLC purity and ESI-MS identity, on a lot-numbered COA.
Net peptide content, not just purity — RP-HPLC + ESI-MS, lot-numbered COA.
Net peptide content on every lot's COA.
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Reading a peptide HPLC trace — a field guide. Read →
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Recombinant erythropoietin protein · heavily regulated
Lyochem buyer fit
This Lyochem page is intentionally written for research labs, core facilities, and method-development teams qualifying EPO (Erythropoietin) as a reference-grade lot. It is not the pharmacy procurement owner for this SKU; the page should win differentiated searches around sequence verification, assay suitability, lot continuity, and data-packet depth.
Overview
As the principal regulator of erythropoiesis, Erythropoietin (EPO, or Epoetin) signals through the homodimeric EPO receptor on erythroid progenitors to drive red-cell production. It is a 165-amino-acid glycoprotein hormone made natively by renal interstitial cells and produced for therapy as a recombinant glycoprotein biologic (Epogen, Procrit, Eprex, NeoRecormon, and numerous biosimilars), with approvals for chemotherapy-associated anemia, anemia of chronic kidney disease, and further indications worldwide. Both its prescription status and its role in athletic-doping enforcement make it one of the most tightly controlled biologicals in cross-border trade. Lyochem releases recombinant EPO exclusively to qualified buyers holding import authorization and dispensing licensing valid in the destination jurisdiction. Activity hinges on glycosylation: an identical protein backbone bearing different glycan structures (Epoetin-alpha, Epoetin-beta, Darbepoetin-alpha, CERA) yields sharply different in vivo pharmacokinetics. Accordingly, the reference-standard release packet layers glycan mapping and isoform analysis on top of standard biologic testing — SDS-PAGE, LC-MS/MS peptide mapping, bioactivity assay, endotoxin, and host-cell-protein.
Applications & buyer fit
Other research peptides in this category — Melanotan-1, Melanotan-2, PT-141, ACE-031, Adipotide-FTTP, EPO, HCG, HMG — ship to research labs studying topics outside the GH / cognitive / immune / mitochondrial / repair / longevity / Khavinson clusters. Each peptide has its own analytical-packet emphasis (e.g. glycoprotein bioassay in IU/mg for HCG; cyclised-form confirmation for oxytocin) noted in the per-product CoA scope.
Contract Research Organisations (CROs)
CROs running preclinical and translational studies on behalf of academic, biotech, or pharma sponsors who need traceable reference material across multi-site campaigns.
Every release ships with its own batch-specific CoA — identity, purity, and the analytical scope agreed at quote stage, tied to the exact lot you receive.
Review a representative batch CoA before you order, so you can confirm the packet matches what your method or sponsor audit needs.
Supplied strictly as a research reagent to research institutions — not a finished dosage form and not for human administration. Buyer qualification runs at the inquiry stage.
Specifications
Documentation available on request
Regulatory note
A biological subject to strict international controls, athletic-doping enforcement among them, and therefore highly regulated. Supply is limited to qualified buyers who hold appropriate licensing in their jurisdiction. Before an order is accepted, a full compliance review and import authorization documentation are required.
Selected literature
Frequently asked questions
Because EPO is a glycoprotein, mass alone is insufficient for identity. The characterization packet combines intact and deglycosylated LC-MS to confirm the 165-residue backbone, peptide mapping (LC-MS/MS after trypsin digest) for sequence coverage, and isoelectric focusing or capillary zone electrophoresis to profile the isoform/glycoform distribution. SDS-PAGE confirms apparent molecular size, and RP-HPLC or SEC assesses purity and aggregation. The isoform banding pattern is the fingerprint that distinguishes recombinant material and separates the different epoetin forms, so buyers qualifying a reference lot should require the electrophoretic isoform trace, not just a single reported mass.
EPO is a cold-chain biological, so handling protects both the protein fold and the sialylated glycans. Keep the lyophilized vial frozen until use, reconstitute gently in the buffer specified on the COA (avoid vortexing, which shears and denatures at the air-liquid interface), and add carrier protein for dilute working stocks to limit surface adsorption at the low concentrations used in bioassay or immunoassay. Aliquot into single-use volumes and minimize freeze-thaw cycles, since repeated cycling drives aggregation and can desialylate isoforms, shifting the electrophoretic pattern and confounding potency or identity readouts against the reference standard.
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